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- RPU52609
- 2025年07月12日
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文献和实验Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation
to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated
of methyl′CpG binding proteins was described 7 . The five members of this family share a similar methyl′CpG binding domain. One of these proteins, MeCP2, is known to be necessary for embryonic development, although the effects on imprinted genes
Methyl DNA Immunoprecipitation
or sequencing, involving time- and labor-intensive chemical treatments that damage DNA and limit throughput. It was shown that the methyl-binding domain (MBD) of MeCP2 has some sequence preference besides its recognition of methyl-CpGs (10 ) and that the MBD
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