Recombinant Cytokeratin 10 (CK

Purification of the Extracellular Domain of the Epidermal Growth Factor Receptor Produced by Recombinant Baculovirus-Infected Insect Cells in a 10-L R

with a recombinant baculovirus that carries a DNA insert encoding for the entire extracellular domain, including the N-terminal signal sequence.

Recombinant expression and SDS

the polyacrylamide gel in a plastic or glass container. Cover the gel with 3 to 5 gel volumes isopropanol fixing solution and shake gently at room temperature for 10 min. 10. Pour out fixing solution. Cover the gel with rapid Coomassie blue staining solution

DETECTION OF RECOMBINANT PROTEINS BY ELISA

of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie