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文献和实验Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA
Phospholipid hydroperoxide glutathione peroxidase (PHGPX or GPX4, E.C. 1.11.1.12) is one of the four identified selenium-dependent glutathione peroxidases (GPX) in mammals (1 ). Both of the pig (2.8 kb) and the mouse (4.0 kb) GPX4 genes
Methods to Detect Protein Glutathionylation
is a posttranslational modification that results in the formation of a mixed disulfide between glutathione and the thiol group of a protein cysteine residue. Glutathionylation of proteins occurs via both nonenzymatic mechanisms involving thiol/disulfide exchange
Studying Protein Interactions by Far-Western Blotting Far-Western blotting was originally developed to screen protein expression libraries with 32P-labeled glutathione S-transferase (GST)-fusion protein. Far-Western blotting is now used to identify
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