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文献和实验一、CA125检测的特征 CA125的测定属肿瘤标志物的检测方法,完全不同于由绒毛分泌的绒毛促性腺激素hCG具有极高的特异性与敏感性,因而需要了解其原理才能很好的应用于临床。肿瘤没有特异性抗原,只有肿瘤相关抗原,因之特异性差。 1981年美国科学家Bast以卵巢上皮癌细胞株(ovarian carcimoma antigen,OVCA433)为抗原制备了单克隆抗体OC125,OC125所识别的抗原称为CA125(carcimoma antigen,CA125
Applications of Lectin Histochemistry and Cytochemistry in Diagnosis and Prognosis
tumor pathology recognize epitopes that include carbohydrate sequences, e.g., α-fetoprotein, carcinoembryonic antigen, CA125, which may also be identified by lectins Cancer-associated oligosaccharides are often oncodevelopmental in nature and reflect
, either by culturing cells with radioactive precursor or by labeling the molecule after synthesis has been completed (e.g., by radioiodination to iodinate tyrosine residues or by sodium [3H]borohydride reduction to label carbohydrate). Having a radioactive antigen
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