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文献和实验Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE
of enzyme-linked immunosorbent assays (ELISAs) and monoclonal antibody technology has proven instrumental for advancing the science of Ig biology. IgE is of interest as it is the primary Ig responsible for allergic reactions ranging from allergic rhinitis
免疫球蛋白 E(immunoglobulin E, IgE)
血清中含量最少,发现最晚的一种抗体,仅占血清中免疫球蛋白总含量的0.001%。这类免疫球蛋白的构形与IgG相似,不耐热,56℃30分钟可灭活。由鼻咽、扁桃体、支气管、胃肠道粘膜等处固有层的浆细胞产生,一般分布在血管外,正常血清中很少。是亲细胞抗体,能与血液中的嗜碱性粒细胞、组织中的肥大细胞结合,导致I型变态反应的发生。骨髓瘤、肝脏疾病、慢性寄生虫病或细菌感染,以及自身免疫病患者,体内IgE含量增加。
Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma
Conventionally, monoclonal antibodies (MAbs) are generated by fusing B cells from an immunized animal with myeloma cells from the same species (1 ). Several murine MAb have already been employed for in vivo diagnosis and therapy, including
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