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- Biomatik
- CAU22335
- 2025年07月12日
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文献和实验Studies of the Ubiquitin Proteasome System
both in vitro and in cells. Because another protein modifier, NEDD8, itself regulates aspects of the ubiquitin system, basic protocols on neddylation are also included in this unit. Keywords: Ubiquitin; ubiquitylation; proteasomal degradation; protein
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
, immunostaining of the chromosomes with a protein of interest, and counterstaining with Giemsa and Hoechst. RELATED INFORMATION Technical modifications incorporated into this protocol that have enabled more reproducible patterns of banding
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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