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- CAU21811
- 2025年07月14日
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文献和实验Methods for use with the mTn-3xHA/GFP-mutagenized library
of the GAL1-10 promoter on galactose. Our strains grow poorly on galactose but give 80 to 100% popouts. The HA triple tag can be detected by mouse monoclonal antibodies 12CA5 (Boehringer) or MMS101R (BAbCo, Richmond, California). These antibody recognise
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
-treatment conditions.Histochem . J. 18 :271. Rodriguez, J., and F. Deinhardt. 1960. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology 12 :316. Rosenberg, W.S., X.O. Breakefield, C. DeAntonio, and O
into cells by mating with strain #111/B428. Grow strains overnight with antibiotic selection (Tet3 for #111/B428). Subculture 1:100 in fresh LB medium (no antibiotics). Grow at 37o C to early log phase (when cell swirls are visible
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