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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
3年
- 英文名:
Blood Agar Base N0.2
- 库存:
999
- 供应商:
北京伊塔生物科技有限公司
- 规格:
250g
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文献和实验of cells and Cancer cells will have higher plating efficiencies than normal cells. Procedure 1. Make 1.2% and 0.6% noble agar medium in dw. 2. Autoclave both noble agar medium amd equilibrate at 42 degree celsius. 3. Prepare 20% FBS containing
SOFT AGAR ASSAY FOR COLONY FORMATION
Note: All volumes are calculated to cater for four plates per point. Base Agar 1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath. Warm 2X RPMI + 20% FCS to 40 in waterbath. Allow at least 30 minutes for temperature
Preparation of Antibiotic Stock Solutions and Agar Plates
of Yeast Extract, 20 g of Agar. 2. Adjust pH to 7,0 (with 5 N NaOH if necessary) . 3. Add deionized Water to a final Volume of 1 Liter. 4. Autoclave (121℃ program for solutions, put autoclave-temperature-sensor into water) . 5. Let it cool
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