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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
3年
- 英文名:
Buffered Sodium Chloride Peptone Solution PH7.0
- 库存:
999
- 供应商:
北京伊塔生物科技有限公司
- 规格:
250g
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文献和实验Measurement of Chloride Movement in Neuronal Preparations
, protocols are described for biochemical and optical techniques that have been used by investigators to measure ligand?gated chloride movement in vesicular structures called synaptoneurosomes (also referred to as microsacs), in cultured neurons
Competent cells, calcium chloride method, E. coli, description
preparation, most of the nucleic acid at this point is RNA). Extract the solution once with 5 ml chloroform:isoamyl solution, centrifuge and save the aqueous layer. Add 0.1 volumes of 3 M Sodium acetate, mix, overlay with 2 volumes of 95% ethanol
母液要分开灭菌不然会有沉淀。在M9培养基基础上添加0.5%葡萄糖即为MM培养基。 2.14 BMGY /BMMY培养基: 酵母粉:1.0克,蛋白胨:2.0克,YNB:1.34克,0.1mol/L pH6.0(或者pH7.0)磷酸缓冲液,甘油:1.0毫升,加蒸馏水到100mL 2.15 BMM培养基,全称:Buffered minimal methanol 即最小缓冲甲醇培养基,常用于表达分泌型蛋白的培养基。 配制: 100mM pH
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