- ￥2500 - 5900
- 齐源
- 656103
- 国产/进口
- 2025年07月07日
- ICFC
- 小鼠
- 人、小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
SOX2
- 级别：
SOX2 Antibody
- 目录编号：
SOX2 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
SOX2 Antibody
- 抗体英文名：
anti-SOX2 Antibody-PE
- 抗体名：
SOX2 Antibody
- 标记物：
PE
- 宿主：
小鼠
- 适应物种：
人、小鼠
- 免疫原：
SOX2 Antibody
- 亚型：
SOX2 Antibody
- 形态：
SOX2 Antibody
- 应用范围：
ICFC
- 保存条件：
2-8℃
- 浓度：
SOX2 Antibody
- 规格：
25 tests/100 tests
| 规格： | 25 tests | 产品价格： | ￥2500.0 |
|---|---|---|---|
| 规格： | 100 tests | 产品价格： | ￥5900.0 |
Description ：SOX2 is the most studied member of SRY-related box transcription factor family. It binds totarget genes through its highly conserved HMG box domain. Inactivation of the SOX2 genecauses lethality during embryonic development. SOX2 knockdown in embryonic stem cellsresults in their differentiation. Co-expression of SOXwith OCT4, MYC, and KLF4 is sufficient toreprogram somatic cells to induced pluripotent stem cells (iPSCs), which exert similarcharacteristics as natural pluripotent stem cells. These findings indicate that SOX2 is crucial forthe self-renewal and pluripotency of embryonic stem cells. In addition, over-expression of SOX2has been found in various types of malignant cancer. Knockdown of SOX2 results in cell cyclearrest by downregulating cyclin D1 and inhibition of tumor cell proliferation, suggesting thatSOX2 is involved in activating genes associated with tumor progression.
Verified Reactivity ：Human, Mouse
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：Full length human SOX recombinant protein
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation ：The antibody was purified by affinity chromatography and conjugated with PE under optimalconditions.
Concentration ：Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：ICFC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by intracellular immunofluorescent staining withflow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl permillion cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser ：Blue Laser (488 nm)、Green Laser (532 nm)、Yellow-Green Laser (561 nm)
Application Notes ：This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determinedby in-house testing).
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文献和实验1. Lee K, et al. 2019. Cell Rep. 28:382. PubMed
2. Radhakrishnan S, et al. 2019. Mol Med Rep. 20:813. PubMed
3. Wang C, et al. 2020. British Journal of Pharmacology. 177(13):3009-3023. PubMed
4. Yang Z, et al. 2020. Sci Rep. 17757:10. PubMed
5. Mirzaei R, et al. 2021. Sci Adv. 7:eabh2148. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低，实验程序少尽量减少实验工序和过程，以保证实验的真实和准确性。因此在条件允许的范围内，建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择： 如果实验中检测单一指标：不同荧光标记在不同的仪器上强度不同。以某仪器为例：PE ＞APC ＞PE-Cy5 ＞PerCP ＞FITC ＞PerCP-Cy5.5，通常来说，PE最强，适用于弱表达抗原。FITC强度较弱，适用于强表达抗原，使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗，这种抗体有单抗的吧？ 用于FCM： 1：20-100，这是说用于流式的稀释倍数，是不是稀释倍数越小越好呢？ 同型对照抗体是不是必须要做的？如何选择阴型同型对照抗体呢！ 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助！ freecell 抗体的稀释倍数是需要优化
有了质量好的抗原，还必须选择适当的免疫途径，才能产生质量好（特异性强和效价高）的抗体。 （一）用于免疫的动物 作免疫用的动物有哺乳类和禽类，主要为羊、马、家兔、猴、猪、豚鼠、鸡等，实验室常用者为家兔、山羊和豚鼠等。动物种类的选择主要根据抗原的生物学特性和所要获得抗血清数量，如一般制备抗r-免疫球蛋白抗血清，多用家兔和山羊，因动物反应良好，而且能够提供足够数量的血清，用于免疫的动物应适龄
