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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD226 (DNAM-1)
- 级别:
CD226 (DNAM-1) Antibody
- 目录编号:
CD226 (DNAM-1) Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
CD226 (DNAM-1) Antibody
- 抗体英文名:
anti-human CD226 (DNAM-1) Antibody-APC
- 抗体名:
CD226 (DNAM-1) 抗体
- 标记物:
APC
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
CD226 (DNAM-1) Antibody
- 亚型:
CD226 (DNAM-1) Antibody
- 形态:
CD226 (DNAM-1) Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
CD226 (DNAM-1) Antibody
- 规格:
25 tests/100 tests
| 规格: | 25 tests | 产品价格: | ¥2100.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥4500.0 |
Description :DNAM-1 (CD226) is a ~65 kD glycoprotein expressed on cell surface of T cells, NK cells,monocytes/macrophages, platelets and megakaryocytes and a subset of B cells and a memberof the immunoglobulin (Ig)-superfamily containing 2 Ig-like domains of the V-set. The ligandsfor CD226 are the poliovirus receptor (CD155) and its family member nectin-2 (CD112), whichare broadly expressed on epithelial, endothelial and neuronal cells. CD226 is physicallyassociated with LFA-1 in NK cells and activated T cells, and involved in LFA-1-mediatedsignaling.
Verified Reactivity :Human
Reported Reactivity :African Green, Baboon, Cynomolgus, Rhesus
Antibody Type :Monoclonal
Host Species :Mouse
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation :The antibody was purified by affinity chromatography and conjugated with APC under optimalconditions.
Concentration :Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysis online tool.)
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser :Red Laser (633 nm)
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文献和实验1. Fuchs A, et al. 2004. J. Immunol. 172:3994
Product Citations:
1. Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed
2. Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
3. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
4. Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed
5. Sutavani R, et al. 2013. J Immunol. 191:5895. PubMed
6. Jacobs ME, et al. 2021. Immunother Adv. 1:ltaa004. PubMed
7. Choi JW, et al. 2019. BMC Cancer. 19:817. PubMed
8. Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
9. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
10. Alonso R, et al. 2022. Blood Adv. :. PubMed
11. Elias S, et al. 2020. Haematologica. . PubMed
5.5, APC等。 常见荧光素波长分布示意图 同型对照的选择: 流式细胞实验和其他抗体相关实验有点不同的就是需要选择同型对照。同型对照,是用于消除由于抗体非特异性结合到细胞表面而产生的背景染色,相当于实验的阴性对照。同型对照选择与标记抗体同种属来源,同亚型,同荧光标记的抗体。比如:抗人的CD3的PE标记的抗体,小鼠的IgG2a。同型
允许的范围内,建议尽量用直接标记的抗体进行实验而不去做间接标记。 3. 流式抗体荧光标记的选择:如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。FACS Calibur仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。如果同时检测多个指标:确认流式细胞仪能检测多少个通道:流式抗体每个通道只能选择1种荧
而不去做间接标记。3 流式抗体荧光标记的选择:如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。FACS Calibur仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。如果同时检测多个指标:确认流式细胞仪能检测多少个通道:流式抗体每个通道只能选择1种荧光素。各个通道之间的荧光素可以随意搭配。如:实验者同时检测三个指标











