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文献和实验, mitochondrial uncoupling proteins (UCPs) displayed (non-native) artefactual uncoupling when expressed in yeast or can possess functions upon reconstitution in proteoliposomes that cannot be reproduced in isolated mitochondria. In the light of newly discovered
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high?throughput manner. This unit describes a protein microarray platform that significantly improves
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