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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
A Homogeneous Assay to Assess GABA Transporter Activity
Corey, J.L., Guastella, J., Davidson, N., and Lester, H.A. 1994. GABA uptake and release by a mammalian cell line stably expressing a cloned rat brain GABA transporter. Mol. Membr. Biol. 11:23‐30.
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