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文献和实验Two-hybrid analysis of genetic
,is expressed from a plasmid containing the HIS3 marker,and the activation domain fused protein,or prey,is expressed from a plasmid containing the TRP1 marker.In the most commonly used bait plasmid,pEG202,the bait is expressed from the constitutive yeast ADH
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
Figure 17.4.1 Photograph of a phage ELISA result. Using eight different clones that were isolated with GST‐SrcSH3 domain as the target, 25 µl of culture supernatant were added to pairs of microtiter plate wells containing GST‐SrcSH3 or GST
Spectroscopic Methods for the Determination of Protein Interactions
Figure 20.8.5 Effect of the fluorescence of compound S on binding data. Simulated data for the addition of S to a solution originally containing 15 µM protein A ( K d = 5 µM, p = 1) are shown. Fluorescence
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