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文献和实验Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
) HRP‐conjugated anti‐flag antibody Tyramide signal amplification (TSA) kit (Life Technologies, cat. no. T30953) containing
Immune Response Biomarker Profiling
and store at 4°C or on ice. 3) Make sure the buffers are cold and stored on ice until use. Place an incubation tray on ice to chill until use. 3. Blocking Step 1) Place the mailer containing the ProtoArray® Human Protein Microarray v
labeled above. The controls used in this experiment were the Pum- (bop-) host strain SD23, strain 100E (SD23 containing plasmid with unmutated promoter) and TATA box mutants (1B2, 2B12, 2H10 and 1D2). A sequencing reaction (C lane) performed
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