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文献和实验Establishment of Stable Transfectant of CHO Lec Cells
medium: To 9 lt of mili-Q water, add MEM w/o Gln (GIBCO, #11700-077). Pwdr for 10 lt.NaHCO3 , 27.75gglucose: 30 gnucleosides: adenosine (Sigma A-4036), 70mg; guanosine (Sigma G-6264), 70mg; cytidine (Sigma C-4654), 70mg; uridine (Sigma U
Establishment of Stable Transfectant of CHO Lec Cells
mg; guanosine (Sigma G-6264), 70mg; cytidine (Sigma C-4654), 70mg; uridine (Sigma U-3003), 70mg; thymidine (Sigma T-1895),24mg glutamic acid (G-1626) and asparagine (A-0884); 600 mg each sodium pyruvate solution (100X, 100mM, GIBCO #11360-021
place through one of two pathways. In one pathway, long dsRNAs activate a protein kinase, PKR. Activated PKR, in turn phoshorylates and inactivates the translation initiation factor, eIF2a, leading to repression of translation. (39) In the other pathway
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