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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
Analysis for Saturation and Competition Assays Basic Protocol 3: Measurement of 5‐HT1A Receptor Binding in Tissue Membrane Homogenates Basic Protocol 4: Quantitative Autoradiography of 5‐HT1A Binding to Rat Brain
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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