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文献和实验Assaying Phospholipase A2 Activity
Mammalian cells contain many structurally and functionally diverse phospholipases A2 (PLA2 ) that catalyze the hydrolysis of sn-2 fatty acid from membrane phospholipid. Assays are described for measuring the activity of Group IVA cytosolic
Phospholipases A2 (PLA2 ) catalyze the hydrolysis of the ester bond at the sn-2 position of phospholipids to generate the lysophospholipid and free fatty acid (1 ). Several classes of PLA2 s have been identified (2 ). They include the low
The release of arachidonic acid (AA) from membrane phospholipids by phospholipase A2 (PLA2 ) is a critical step in the generation of lipid mediators by mast cells. AA released by PLA2 activity is metabolized to bioactive compounds
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