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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
as well as single strand breaks ("nicks") in high molecular weight DNA can be identified by labeling free 3’-OH termini with modified nucleotides in an enzymatic reaction. Terminal deoxynucleotidyl transferase (TdT), which catalyzes polymerization of nucleotides
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
High‐molecular‐weight human genomic DNA, prepared from peripheral blood using commercial kit (e.g., Puregene; Gentra Systems) 10 U/µl restriction endonucleases (Taq
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