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文献和实验Characterization and Validation of Cre‐Driver Mouse Lines
are increasingly popular strategies in biomedical research. These approaches rely on the production of conditional genetically engineered mutant mouse (GEMM) lines with mutations in protein?encoding genes. These conditional GEMMs are then bred
A Comprehensive Guide to Sleeping Beauty–Based Somatic Transposon Mutagenesis in the Mouse
. Mouse Biol. 1:347?368 © 2011 by John Wiley & Sons, Inc. Keywords: Sleeping Beauty; cancer; mutagenesis screen; transposon; mouse GO TO THE FULL PROTOCOL:
Use of Internaland External Standardsor Reference RNAs for A
, respectively, in a MAXIscript in vitro transcription reaction. 4 x 104 cpm of each probe was added to 5 µg total RNA from different mouse tissues and processed in a multiple probe ribonuclease protection assay using the RPA II Kit. Results show a comparison
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