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文献和实验Procedures for In Vitro Production of Bovine Embryos
in preimplantation bovine embryos. Mol Reprod Dev 1997; 48: 25-33.Hernandez-Ledezma JJ, Sikes JD, MurpheyCN, Watson AJ, Schultz GA, Roberts RM. Expression of bovine trophoblast interferon in conceptuses derived by in vitro techniques. Biol Reprod 1992; 47: 374-380
.Scale down the volume for the "microassay procedure," which uses 1 ml cuvettes.Protocols,including use of microtiter plates are described in the flyer that comes with the Bio-Rad kit.PrincipleThe assay is based on the observation that the absorbance
. The Bradford assay is rather sensitive to bovine serum albumin, more so than "average" proteins, by about a factor of two. Immunoglogin G (IgG - gamma globulin) is the preferred protein standard. The addition of 1 M NaOH was suggested by Stoscheck (1990
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