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文献和实验Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
are produced as soluble recombinant proteins by transiently transfecting a mammalian cell line. The proteins are purified using an oligo‐His tag using a bespoke purification apparatus (the “Protein Press”) and subsequently printed onto glass slides
【资源】[共享]Adhesion Protein Protocols——(很有用的)
1— Introduction 2— Materials 2.1— Cell-Culture Media 2.2— Materials for Culture of Human Endothelial Cells on Porous Filters 3— Methods 3.1— Culture of Human Endothelial Cells on Porous Filters According to the Method of Langeler
的原理用于分子诊断方面:TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的,相关文章见:1,Rapid screen for truncating ATM mutations by PTT-ELISA . 2,An ELISA-based high throughput protein truncation test
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