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- 2025年07月13日
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文献和实验Genotyping for Cytochrome P450 Polymorphisms
Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand
. This method is based on the conversion of the primary cytochrome P450 metabolite, 7α-hydroxycholesterol, into 7α-hydroxy-4-cholesten-3-one in a reaction catalyzed by exogenous cholesterol oxidase, followed by chromatographic separation with monitoring at 254
253322.1|AF253322 Homo sapiens cytochrome P450 (CYP1A1) and cytochrome P450 (CYP1A2) genes, complete cds Length = 43064 Score = 44.1 bits (22), Expect = 0.013 Identities = 22/22 (100%) Strand = Plus / Plus Query: 21 ttaggagtcttgtctcatgcct 42
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