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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
), and for validation of candidate GAP?modulatory molecules with the single?turnover GTP hydrolysis assay. Curr. Protoc. Pharmacol. 56:2.8.1?2.8.15. © 2012 by John Wiley & Sons, Inc. Keywords: regulator of G?protein signaling (RGS) proteins
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
described in this protocol highlight the use of this assay in the quantification of the affinity of binding partners of the regulator of G?protein signaling protein, RGS19, in either a saturation or a competition format. An adaptation of this method
TRAF‐Mediated TNFR‐Family Signaling
of the protein kinase RIP to the TNF receptor‐1 signaling complex. Immunity 4:387‐396. Hsu, H., Shu, H.B., Pan, M.G., and Goeddel, D.V. 1996b. TRADD‐TRAF2 and TRADD‐FADD interactions
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