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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
【分享】Human press METHODS IN MOLECULAR BIOLOGY
.Ubiquitin-Proteasome Protocols Edited by Patterson, Cam 2005 302.Handbook of ELISPOT Methods and Protocols Edited by Kalyuzhny, Alexander E. 2005 303.NanoBiotechnology Protocols Edited by Rosenthal, Sandra J 2005 304.Human Retrovirus Protocols Virology
的原理用于分子诊断方面:TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的,相关文章见:1,Rapid screen for truncating ATM mutations by PTT-ELISA . 2,An ELISA-based high throughput protein truncation test
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