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文献和实验Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
protein discovery initiative (SPDI), a large‐scale effort to identify novel human secreted and transmembrane proteins: A bioinformatics assessment. Genome Res. 13:2265‐2270.
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
DNA purification kit (e.g., QIAquick Spin Miniprep Kit, Qiagen; optional) 2.5 U/µl proofreading PCR polymerase (e.g., Deep Vent; New England Biolabs) and 10× buffer
Basic Protein Sequence Analysis
for Understanding Results of Predictions of Helical Transmembrane Regions and Subcellular Localization Alternate Protocol 3: Predicting the Subcellular Localization of a Protein Using Targetp Support Protocol 5: Guidelines
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