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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
Procedures for In Vitro Production of Bovine Embryos
, Chenoweth PJ, Hansen PJ. Deleterious actions of gossypol on bovine spermatozoa, oocytes and embryos. Biol Reprod 1997; 57, 901-907.Edwards JL, Ealy AD, Monterroso VH, Hansen PJ. Ontogeny of temperature-regulated heat shock protein 70 synthesis
tag on either the sense or antisense strand (City of Hope Protein and synthesis core facility) and various iterations were generated by first heating the RNAs (sense or antisense +/- biotin) to 70°C for 5 minutes and then mixed together and room
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