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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
) Incubate at 70°C for 30 min. ii. Purify using MinElute PCR Purification Kit or phenol:chloroform extraction. iii. Elute with or resuspend in 10 µL of 1X TE, pH 7.4. Alternatively, Klenow
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
will need 100 µL of this bead mix. The choice of beads will strictly depend on the type of antibody being used. ii. Add 1 mL of 0.5% BSA in PBS and mix. iii. Place the tube in a magnetic stand for 1 min
and enzymes that harbors the ability torecognize and cleave foreign nucleic acids based on their sequencesignature. Three known CRISPR systems, types I, II, and III, havebeen described so far [ 12 – 15 ]. Among these different CRISPRsystems, type II CRISPR
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