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文献和实验Crystallization of Kinesin Family Motor Proteins
Tris pH 7.9, 5 mM MgCl2, 0.5 mM ADPReservoir: 17.5% PEGMME 2000, 100 mM HEPES pH 6.5-7.5, 3% glycerolSong et al., 2001Kar3 +N11 (WT)Hanging Drop 18°C2 microliters of protein (15 mg/ml) + 2 microliters of well solution containing 20-26% PEG2000ME, 0.2 M
that encodes a hybrid transcription factor (Fig. 1A). The hybrid transcription factor consists of two domains: a DNA-binding domain and a transcriptional activation domain. We used the Gal4 transcriptional activation domain fused to the Zif268 protein
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
High‐molecular‐weight human genomic DNA, prepared from peripheral blood using commercial kit (e.g., Puregene; Gentra Systems) 10 U/µl restriction endonucleases (Taq
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