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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
can be effectively homogenized by pipetting up and down or vortexing after addition of Cell lysis buffer. 3. Place the tube containing the homogenates on the bench at room temperature for 2 minutes. 4. Add 100 μL of Protein Precipitation Reagent
Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
Abrogation of the RB-p16Tumor Suppressor Pathway in Human Lung Cancer
There is compelling evidence that human lung cancers are characterized by disruption of several important physiological pathways that govern proliferation, apoptosis, intracellular signaling, and cell-cell interactions. Uncontrolled cellular
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