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文献和实验PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
for most in vitro studies of alveolar epithelial function (e.g., transport and various metabolic pathways). The primary culture of human alveolar epithelial cells involves isolation, purification, and culture of alveolar epithelial type II (ATII) cells from human
The Application of the Chemical Array for Biological Study
library GLORIA (Gene Library of Osada Laboratory at RIKEN for chemical array analysis) has been established. Using the systematic platform, we detected novel inhibitors of carbonic anhydrase II. We also have shown that this screening method is useful
Analysis of Proteins using Small Format 2D Gel Electrophoresis
(obtained from Pharmacia Biotech): bovine serum albumin (67 kDa), ovalbumin (43 kDa), carbonic anhydrase (30 kDa) and trypsin inhibitor (20.1 kDa). Post-electrophoretic identification of proteins Various protocols
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