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文献和实验Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections
, including osteocalcin, osteopontin, osteonectin, bone sialoprotein, decorin, and biglycan. Of this group only osteocalcin and bone sialoprotein are specific for bone, whereas the other proteins are also present in other, noncalcifying, tissues. The knowledge
: bisearch.enzim.hu, BSP相关primer design, 挺好, 最重要的是你可以search whole genome (human, mice, etc.)来确认非特异的结合和产物, 因为大部分C都转化成T之后, 非特异的可能性大大增加. (此处非广告)还有, touch down PCR对BSP很好用(对所有有false priming问题的PCR应该都有用), 我一般从TA高几度开始,到低5度结束, 效果很好.4. sequencin我是把PCR产物装到invitrogen的TA
Human mast cells (HMCs) are derived from a CD34+ pluripotent progenitor cell that is Kit (CD117+), CD13+, FcεRI- and lacks lineage-specific surface markers. Bone marrow and peripheral blood are two tissue sources available for obtaining CD
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