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文献和实验Osteoclast Formation in the Mouse Coculture Assay
, osteoclasts can be identified by tartrate-resistant acid phosphatase (TRAP) staining, and, when the cultures are performed on dentine slices, resorption activity can be measured as well. Even though today it is possible to generate osteoclasts from bone marrow
Analysis of Osteoclast Function in Mouse Calvarial Cultures
can produce pits (8 ), and two distinct morphologies of multinucleate tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like cells have been described with differing resorptive capacities (9 ). Like the osteoclast, the foreign body giant cell
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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