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Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the Raclure Method
-inhibited, can be grown in culture for about 20–25 passages, exhibit an immuno-phenotype similar to bone marrow MSCs (negative for CD34 and CD45, but positive for CD44, CD71, CD73, CD105, CD166, HLA ABC, and SSEA-4), and can differentiate into osteocytes
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the ISCT minimal defining criteria for human MSCs, namely adherence to plastic, a surface antigen expression profile of CD29+, CD44+, CD49a+ CD49e+, CD73+, CD105+, CD166+, CD34−, CD45−, and a differentiation potential that includes adipogenesis
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