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文献和实验Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the Raclure Method
-inhibited, can be grown in culture for about 20–25 passages, exhibit an immuno-phenotype similar to bone marrow MSCs (negative for CD34 and CD45, but positive for CD44, CD71, CD73, CD105, CD166, HLA ABC, and SSEA-4), and can differentiate into osteocytes
Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells
the ISCT minimal defining criteria for human MSCs, namely adherence to plastic, a surface antigen expression profile of CD29+, CD44+, CD49a+ CD49e+, CD73+, CD105+, CD166+, CD34−, CD45−, and a differentiation potential that includes adipogenesis
Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
Mesenchymal stem cells (MSCs) have been isolated from many tissues including differentiating human embryonic stem cells (hESCs). Derivation of MSCs from hESCs consists of two major steps: differentiation and isolation. In our hands
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