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| E Binding Buffer | 博日 | BSC14S03 | / | / | 定制 | / | 50.0 | / | / | BSC14S1配套组分 |
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文献和实验| E Binding Buffer | 博日 | BSC14S03 | / | / | 定制 | / | 50.0 | / | / | BSC14S1配套组分 |
Preparation of Microinjection Buffer
Buffer composition is 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 30 microM spermine, 70 microM spermidine, 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact, unfragmented DNA molecules. See the following references: Schedl
Pouring Linear and Buffer-Gradient Sequencing Gels
to be read from a single run) up to 100 cm in length can be poured, as can wider gels (to enable more clones to be loaded onto a single gel). However, these larger gels are more difficult to handle after electrophoresis, i.e., during fixation, drying
Screening Peptide/Protein Libraries Fused to the Repressor DNA-Binding Domain in E. coli Cells
The use of λ repressor fusions to study protein-protein interactions in E. coli was first described by Hu and others ( 1 ). Since then, the repressor system has been employed by several laboratories to screen
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