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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
MIF
- 级别:
anti-human MIF Antibody
- 目录编号:
anti-human MIF Antibody
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
anti-human MIF Antibody
- 抗体英文名:
Purified anti-human MIF Antibody
- 抗体名:
MIF 抗体
- 标记物:
anti-human MIF Antibody
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
anti-human MIF Antibody
- 亚型:
anti-human MIF Antibody
- 形态:
anti-human MIF Antibody
- 应用范围:
ELISA Capture, WB
- 保存条件:
2-8℃
- 浓度:
0.5 mg/ml
- 规格:
50 ug/500 ug
| 规格: | 50 ug | 产品价格: | ¥2100.0 |
|---|---|---|---|
| 规格: | 500 ug | 产品价格: | ¥6100.0 |
Description :Human macrophage migration inhibitory factor (MIF) is a 12.5 kD, 115 amino acid, nonglycosylated polypeptide expressed by multiple cell types, including activated T cells,macrophages, eosinophils, epithelial cells, and endothelial cells. MIF plays many roles inbiological processes such as catalytic activity, immunity, endocrine regulation, signalmodulation, and inflammation.
MIF is expressed in malignant cells including lung, liver, breast, colon, and prostate tumors.Studies suggest that MIF might serve as a molecular link between chronic inflammation andcancer
Recombinant MIF consists of a mixture of monomers, dimers, and trimers. The physiologicallyactive forms are believed to be predominantly dimeric and trimeric forms.
Verified Reactivity :Human
Antibody Type :Monoclonal
Host Species :Mouse
Immunogen :Recombinant human MIF with a His tag
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography.
Concentration :0.5 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C.
Application :ELISA Capture - Quality tested、WB - Verified
Recommended Usage :Each lot of this protein is quality control tested by ELISA assay. A concentration of 4 µg/ml of thecapture antibody was utilized to generate the example standard curve. It is recommended that eachlot of reagent be titrated for optimal performance for each application.
Application Notes :ELISA: To measure human MIF, the purified 10C3 antibody (Cat. No. 525501) is useful as thecapture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 10C3antibody (Cat. No. 525503) as the detection antibody.
Additional reported applications (for the relevant formats) include: functional assay andneutralization . The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) isrecommended for functional assays (contact our custom solutions team).
Note: For testing human MIF in serum, plasma or cell culture supernatant, BioLegend's LEGEND-MAX™ ELISA Kits (Cat. No. 438407 & 438408) are specially developed and recommended.
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