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- 文献和实验
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- 抗体英文名：
Anti-alpha-Synuclein Antibody, Rabbit Polyclonal
- 抗体名：
Anti-alpha-Synuclein Antibody, Rabbit Polyclonal
- 规格：
100 µL
Anti-alpha-Synuclein Antibody, Rabbit Polyclonal 产品信息
产品名称Anti-alpha-Synuclein Antibody, Rabbit Polyclonal
经验证的应用WB (FAQProtocol), ELISA (FAQProtocol), IHC-P (FAQProtocol), ICC/IF (FAQProtocol), IP (FAQProtocol)
交叉反应Reacts with: Human
特异性Human alpha-Synuclein
免疫原Recombinant Human Alpha-Synuclein Protein (Catalog#12093-HNAE)
制备方法Produced in rabbits immunized with purified, recombinant Human Alpha-Synuclein (rh Alpha-Synuclein; Catalog#12093-HNAE; P37840-1; Met1-Ala140). Alpha-Synuclein specific IgG was purified by Human Alpha-Synuclein affinity chromatography.
来源Polyclonal Rabbit IgG
纯化Protein A & Antigen Affinity
缓冲液0.2 μm filtered solution in PBS
偶联物Unconjugated
状态Liquid
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-alpha-Synuclein Antibody, Rabbit Polyclonal 经验证的应用
应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
ICC-IF 1:50-1:200
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.
图注：
Anti-SNCA rabbit polyclonal antibody at 1:500 dilution
Lane A: HeLa Whole Cell Lysate
Lane B: 293T Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:14 kDa
Observed band size:16 kDa
图注：Immunochemical staining of human alpha-Synuclein in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
图注：
Immunofluorescence staining of alpha-Synuclein in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human alpha-Synuclein polyclonal antibody (dilution ratio 1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
图注：
SNCA was immunoprecipitated using:
Lane A:0.5 mg SHSY5Y Whole Cell Lysate
Lane B:0.5 mg HeLa Whole Cell Lysate
Lane C:0.5 mg 293T Whole Cell Lysate
4 µL anti- SNCA rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti- SNCA rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14kDa
Observed band size :15 kDa
Anti-alpha-Synuclein Antibody, Rabbit Polyclonal: 别称
Anti-NACP Antibody; Anti-PARK1 Antibody; Anti-PARK4 Antibody; Anti-PD1 Antibody
alpha-Synuclein 背景信息
Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N- and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich. Normally the unstructured soluble type of alpha-synuclein can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. SNCA abnormality and mitochondrial deficiency are two major changes in the brain of patients with Parkinson's disease (PD). Besides, alpha-synuclein is an abundant component of Lewy bodies in sporadic Parkinson's disease and diffuse Lewy body disease.
全称
synuclein, alpha (non A4 component of amyloid precursor)
产品名称Anti-alpha-Synuclein Antibody, Rabbit Polyclonal
经验证的应用WB (FAQProtocol), ELISA (FAQProtocol), IHC-P (FAQProtocol), ICC/IF (FAQProtocol), IP (FAQProtocol)
交叉反应Reacts with: Human
特异性Human alpha-Synuclein
免疫原Recombinant Human Alpha-Synuclein Protein (Catalog#12093-HNAE)
制备方法Produced in rabbits immunized with purified, recombinant Human Alpha-Synuclein (rh Alpha-Synuclein; Catalog#12093-HNAE; P37840-1; Met1-Ala140). Alpha-Synuclein specific IgG was purified by Human Alpha-Synuclein affinity chromatography.
来源Polyclonal Rabbit IgG
纯化Protein A & Antigen Affinity
缓冲液0.2 μm filtered solution in PBS
偶联物Unconjugated
状态Liquid
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-alpha-Synuclein Antibody, Rabbit Polyclonal 经验证的应用
应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
ICC-IF 1:50-1:200
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.
图注：
Anti-SNCA rabbit polyclonal antibody at 1:500 dilution
Lane A: HeLa Whole Cell Lysate
Lane B: 293T Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:14 kDa
Observed band size:16 kDa
图注：Immunochemical staining of human alpha-Synuclein in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
图注：
Immunofluorescence staining of alpha-Synuclein in SHSY5Y cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human alpha-Synuclein polyclonal antibody (dilution ratio 1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
图注：
SNCA was immunoprecipitated using:
Lane A:0.5 mg SHSY5Y Whole Cell Lysate
Lane B:0.5 mg HeLa Whole Cell Lysate
Lane C:0.5 mg 293T Whole Cell Lysate
4 µL anti- SNCA rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Primary antibody:
Anti- SNCA rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14kDa
Observed band size :15 kDa
Anti-alpha-Synuclein Antibody, Rabbit Polyclonal: 别称
Anti-NACP Antibody; Anti-PARK1 Antibody; Anti-PARK4 Antibody; Anti-PD1 Antibody
alpha-Synuclein 背景信息
Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N- and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich. Normally the unstructured soluble type of alpha-synuclein can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. SNCA abnormality and mitochondrial deficiency are two major changes in the brain of patients with Parkinson's disease (PD). Besides, alpha-synuclein is an abundant component of Lewy bodies in sporadic Parkinson's disease and diffuse Lewy body disease.
全称
synuclein, alpha (non A4 component of amyloid precursor)
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文献和实验该产品被引用文献
参考文献
Arima K, et al. (1998) Immunoelectron-microscopic demonstration of NACP / alpha-synuclein-epitopes onthe filamentous component of Lewy bodies in Parkinson's disease and in dementia with Lewy bodies. Brain Res. 808 (1): 93-100.
Arima K, et al. (1998) NACP / alpha-synuclein immunoreactivity in fibrillary components of neuronal and oligodendroglial cytoplasmic inclusions in the pontine nuclei in multiple system atrophy. Acta Neuropathol. 96 (5): 439-44.
Lee HJ, et al. (2001) Membrane-bound alpha-Synuclein Has a High Aggregation Propensity and the Ability to Seed the Aggregation of the Cytosolic Form. The Journal of Biological Chemistry. 277: 671-8.
Arima K, et al. (1998) Immunoelectron-microscopic demonstration of NACP / alpha-synuclein-epitopes onthe filamentous component of Lewy bodies in Parkinson's disease and in dementia with Lewy bodies. Brain Res. 808 (1): 93-100.
Arima K, et al. (1998) NACP / alpha-synuclein immunoreactivity in fibrillary components of neuronal and oligodendroglial cytoplasmic inclusions in the pontine nuclei in multiple system atrophy. Acta Neuropathol. 96 (5): 439-44.
Lee HJ, et al. (2001) Membrane-bound alpha-Synuclein Has a High Aggregation Propensity and the Ability to Seed the Aggregation of the Cytosolic Form. The Journal of Biological Chemistry. 277: 671-8.
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Anti-alpha-Synuclein Antibody, Rabbit Polyclonal
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