检测疫苗免疫后血清中和抗体的表达水平。
● 检测方案:
通过Elisa的方法检测血清样本中的IgG和IgM表达水平,以此检测血清中抗体的表达水平。
● 客户提供样品要求:
a. 样本类型:血清样本,干冰运输;
b. 样本体积:100 μL(可特殊定制);
c. 样本要求:无菌,乙肝、丙肝、HIV、梅毒、EB、CMV检测阴性。
血清总抗体检测数据展示:
图3. AAV9型血清样本总抗体Elisa检测数据示意。
AAV血清型检测 |
AAV1 |
AAV1/2 |
AAV2 |
AAV2-7m8 |
AAV oligo001 |
AAV5 |
AAV6 |
AAV6m |
AAV6.2 |
AAV7 |
AAV8 |
AAV9 |
rAAV2-retro |
AAV-PHP.eB |
AAV-B10 |
AAV-PHP.S |
AAV-PAN |
AAV-DJ |
AAV-ShH10 |
AAV-ShH10(Y445F) |
AAV-Rh10 |
AAV-Anc80L65 |
AAV-BR1 |
AAV-BI30 |
AAV-SCH9 |
AAV MaCPNS1 |
AAV MaCPNS2 |
mac |
AAV DSS |
|
更多血清型请咨询 |
我司优势:● 高灵敏度 ● 高特异性 ● 高通量 ● 高准确性
各类检测用AAV生产
基因:Luciferase/EGFP/mCherry |
AAV1 |
AAV1/2 |
AAV2 |
AAV2-7m8 |
AAV oligo001 |
AAV5 |
AAV6 |
AAV6m |
AAV6.2 |
AAV7 |
AAV8 |
AAV9 |
rAAV2-retro |
AAV-PHP.eB |
AAV-B10 |
AAV-PHP.S |
AAV-PAN |
AAV-DJ |
AAV-ShH10 |
AAV-ShH10(Y445F) |
AAV-Rh10 |
AAV-Anc80L65 |
AAV-BR1 |
AAV-BI30 |
AAV-SCH9 |
AAV MaCPNS1 |
AAV MaCPNS2 |
mac |
AAV DSS |
|
更多其他血清型病毒生产请咨询 |
参考文献
1. Schulz M, Levy DI, Petropoulos CJ, Bashirians G, Winburn I, Mahn M, Somanathan S, Cheng SH, Byrne BJ: Binding and neutralizing anti-AAV antibodies: Detection and implications for rAAV-mediated gene therapy. Mol Ther 2023, 31(3):616-630.
2. Mendell JR, Connolly AM, Lehman KJ, Griffin DA, Khan SZ, Dharia SD, Quintana-Gallardo L, Rodino-Klapac LR: Testing preexisting antibodies prior to AAV gene transfer therapy: rationale, lessons and future considerations. Mol Ther Methods Clin Dev 2022, 25:74-83.
3. Li C, Samulski RJ: Engineering adeno-associated virus vectors for gene therapy. Nat Rev Genet 2020, 21(4):255-272.