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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD45.2
- 级别：
anti-mouse CD45.2 Antibody
- 目录编号：
anti-mouse CD45.2 Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-mouse CD45.2 Antibody
- 抗体英文名：
Alexa Fluor® 700 anti-mouse CD45.2 Antibody
- 抗体名：
CD45.2 Antibody
- 标记物：
anti-mouse CD45.2 Antibody
- 宿主：
小鼠
- 适应物种：
小鼠
- 免疫原：
anti-mouse CD45.2 Antibody
- 亚型：
anti-mouse CD45.2 Antibody
- 形态：
anti-mouse CD45.2 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
25 ug/100 ug
| 规格： | 25 ug | 产品价格： | ￥1700.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥3900.0 |
Description ：CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR,BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kDglycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets.There are multiple isoforms in the mouse that play key roles in TCR and BCR signaltransduction. These isoforms are very specific to the activation and maturation states of thecell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4,TCR, CD22, and Thy-1.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：B10.S mouse thymocytes and splenocytes
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700under optimal conditions.
Concentration ：0.5 mg/m
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highlyrecommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Priorto using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flowcytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Excitation Laser ：Red Laser (633 nm)
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文献和实验1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations：
1. Basu S, et al. 2013. J Immunol Methods. 396:163. PubMed
2. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
3. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
4. Chandran S, et al. 2020. Front Immunol. 11:787. PubMed
5. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
6. Vella JL, et al. 2021. Life Sci Alliance. 4:. PubMed
7. Su Y, et al. 2022. J Hematol Oncol. 15:99. PubMed
8. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
9. Zinselmeyer B, et al. 2013. J Exp Med. 210:757. PubMed
10. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
11. Page N, et al. 2018. Immunity. 48:937. PubMed
12. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
'）2 Fragment（Alexa Fluor® 555 Conjugate）#4413 Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 647 Conjugate）#4414 抗小鼠： Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4408 Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor
分泌。 随后进行表面染色：先以Fc阻断剂（2.4G2克隆，5 μg/mL）处理10分钟，再于4°C避光孵育15分钟，使用含1% BSA和0.1% NaN₃的DPBS缓冲液配制抗体，常用组合包括CD90.2、CD4、CD8、CD25、CD11b和CD45.2等标记。 固定步骤采用2%甲醛（FA，由含<3%甲醇的4% Roti-Histofix稀释），室温孵育40–60分钟。破膜与胞内染色使用0.1%皂苷缓冲液或1×Perm缓冲液，加入抗FoxP3、T-bet、RORγt、IL-17A或IFN-γ等抗体，4°C
光谱，并找到随附的缩窄滤光片，可避免主要的峰值自发荧光。这样做可以大大提升信噪比。 选择量子效率较高的染色剂，或选用浓度更高的造影剂进行实验，可以加强信号，尽管成本更高。现代策略使用近红外（NIR）激发光和 700 纳米以上可激发的标签（如 Cy7 或 Alexa Fluor 750），以避开常见自发荧光体的激发和发射范围（图 2）。 图 2.相对于生物成像中常见的自发荧光分子，Alexa 商业荧光染料的发射峰值。选择在近红外范围内激发和发射的造影剂可以避免自发荧光促成因素造成的不必要的发射。
