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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
+4°C (gel packs)
- 保质期:
Upon receipt, the medium should be stored in the dark at 4°C. Avoid repeated freeze/thaw cycles. Do not exceed 37°C. When stored in the dark at 4°C, the product is stable for six months after first thaw.
- 英文名:
Thaw Medium 12
- 库存:
1
- 供应商:
BPS Bioscience Inc.
- CAS号:
/
- 规格:
100 ml
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文献和实验Fluorescence Mounting Medium (Antifade)
. pH paper of range 6.5-10.0 should be used to check the pH of the medium after addition of 12 drops of the Carb-Bicarb buffer and stirring. Additional drops of buffer are added untill the desired pH is reached. Aliquot the mounting medium
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
.Remove medium from cells and replace with PC12 Transfection Media (10 ml/100mm plate).Place in 5% CO2 to equilibrate, for approx. 1h (meanwhile, make CaP ppt).Ca2PO4 PRECIPITATION:Thaw DNA and 2x HeBS just to room temperature.Calculate amount of DNA
A semi-permanent mounting medium for immunofluorescence microscopy
Materials 6gm glycerol 2.4gm mowiol 6ml distilled water 12ml Tris buffer 0.2M at pH 8.5 Method Weigh out the glycerol in a 50ml plastic tube and add the mowiol. Stir thoroughly. Add water and leave for 2hr
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