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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
-20°C. Stable for at least 6 months from date of receipt when stored as directed.
- 英文名:
2X KRAS Nucleotide Exchange Buffer
- 库存:
1
- 供应商:
BPS Bioscience Inc.
- CAS号:
/
- 规格:
2 x 5 ml
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文献和实验of exonucleases that will, over time, degrade the nucleotide overhangs, reducing the efficiency of the cloning event. While it is not recommended, some PCR products have been successfully cloned after 1 week of storage at +4℃.Importance of Positive and Negative
DNA priming reaction x ul DNA 2 ul Reaction buffer, minus DTT 1 ul primer (20 ng) 10 ul total Heat 90-100℃ 2 min. Cool room temp. 30 min. Enzyme mix 4 ul radioactive nucleotide (12 l) 2 ul reaction buffer ( 6 l) 4 ul water
.7µL -2.10x PCR Buffer* 2.5µL 1x3.dNTPs mix (25 mM each nucleotide) 0.2µL 200 µM (each nucleotide)4.primer mix (25 pmoles/µL each primer) 0.4µL 0.4 µM (each primer)5.Taq DNA polymerase (native enzyme) 0.2µL 1 Unit/25 µL6.genomic DNA template (100 ng/µL
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