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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C or -80°C
- 保质期:
At least 6 months from date of receipt, when stored as directed
- 英文名:
Histone H3 peptide (1-21), Trimethyl K9, Biotin-labeled Recombinant
- 库存:
1
- 供应商:
BPS Bioscience Inc.
- CAS号:
/
- 规格:
500 µl
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文献和实验for detection of H3K27me3+, flag-tagged DNMT3A, siRNA EF52 and the targeted EF1A promoter. Methodology is shown for performing first a ChIP for H3K27me3+ and then a Flag-immunoprecipitation followed by a biotin/avidin pulldown for biotin labeled siRNAs
Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
PTMs and their effectors play in biological processes. An important component of this understanding will come through identification of histone PTM binding proteins. To this end, the peptide pull-down (PPD) assay provides a simple and effective tool
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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