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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80°C (dry ice)
- 保质期:
At least 6 months at -80°C.
- 英文名:
TFRC, Avi-Tag, Fc Fusion(lgG1), Biotin-Labeled Recombinant
- 库存:
1
- 供应商:
BPS Bioscience Inc.
- 规格:
25 µg
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文献和实验Recombinant fusion proteins incorporating experimental protein domains fused to immunoglobulin Fc regions have become widely utilized in studies of protein–ligand interactions. The advantages of these systems include an inherent increase
Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins
for the routine purification of such histidine-tagged fusion proteins. As with any purification regime, complications with IMAC can arise. Lacking the absolute specificity of a biological ligand/ligate system such as the avidin/biotin interaction or an antibody
or polyhistidine-tagged recombinantly expressed bait proteins are often detected with a primary or enzyme-labeled antibody specific to the tag. Antibodies to both these popular fusion tags are commercially available. When recombinant techniques cannot be used
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