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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80°C (dry ice)
- 保质期:
At least 6 months at -80°C.
- 英文名:
CDK13/Cyclin K, GST-Tags Recombinant
- 库存:
1
- 供应商:
BPS Bioscience Inc.
- 规格:
5 µg
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文献和实验Purification of GST Fused Proteins
to 1 lt terrific/K2 K (see appendix) with 0.5 ml 100mg/ml Amp. Grow at 37°C to OD600 of approximately 0.5-0.8 (~90-150 min) Induce with 0.5ml 50mg/ml 1PTG prepared freshly in DDW. Incubate 2-3 hrs 37°C for stable proteins, 1 hr 37°C
Site‐Specific Protein Labeling with SNAP‐Tags
. 2011. Chemical tags for labeling proteins inside living cells. Acc. Chem. Res. 44:784–792. Kamiya, M. and Johnsson, K. 2010. Localizable and highly sensitive calcium
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
The use of affinity tags and especially histidine tags (His-tags) has become widespread in molecular biology for the efficient purification of recombinant proteins. In some cases, the presence of the affinity tag in the recombinant protein
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