- 询价
- BPS Bioscience
- 美国
- 100348-1
- 2025年10月01日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80°C (dry ice)
- 保质期：
At least 6 months at -80°C.
- 英文名：
CD83, Fc-fusion (IgG1), Avi-Tag, Biotin labeled Recombinant
- 库存：
1
- 供应商：
BPS Bioscience Inc.
- 规格：
25 µg
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文献和实验2. Lechmann, M. et al., Trends in Immunology. 2002 Jun; 23(6): 273-275.
Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins
for the routine purification of such histidine-tagged fusion proteins. As with any purification regime, complications with IMAC can arise. Lacking the absolute specificity of a biological ligand/ligate system such as the avidin/biotin interaction or an antibody
Affinity Purification of Proteins Binding to GST Fusion Proteins
and the complex is then used to assay the binding of a specific test protein that has been labeled with [35 S]methionine by in vitro translation. However, this method can be adapted for use with other types of fusion proteins; for example, His6 , biotin tags
or polyhistidine-tagged recombinantly expressed bait proteins are often detected with a primary or enzyme-labeled antibody specific to the tag. Antibodies to both these popular fusion tags are commercially available. When recombinant techniques cannot be used
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