- 询价
- BPS Bioscience
- 美国
- 101914
- 2025年12月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80
- 保质期：
6个月
- 英文名：
PI3 Kinase p110α (H1047L)/p85α, FLAG-Tag Recombinant
- 供应商：
BPS Bioscience
- 规格：
1 vial
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文献和实验Identification of a Mutant Kinase/ATP Analog Pair
, it is necessary to screen ATP analogs for their compatibility with the kinase mutant. This step requires an active form of the wild-type and mutant kinase, either as recombinant activated protein, or as protein that has been immunoprecipitated from transfected
the appropriate quantity of Sepharose CL-6B beads (30 µl/sample) with hypotonic buffer and mix with FLAG-M2 agarose beads (10 µl/sample), or beads conjugated to another antibody to the chosen epitope tag (~1 µg of antibody per 1 mg of protein lysate).
同位素法测定底物磷酸化活性方法 Phosphorylation of Substrates
quantity of Sepharose CL-6B beads (30 µl/sample) with hypotonic buffer and mix with FLAG-M2 agarose beads (10 µl/sample), or beads conjugated to another antibody to the chosen epitope tag (~1 µg of antibody per 1 mg of protein lysate).
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