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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80°C (dry ice)
- 保质期:
At least 6 months at -80°C.
- 英文名:
EZH2 / EED / SUZ12, His-Tag, FLAG-Tag Recombinant
- 库存:
1
- 供应商:
BPS Bioscience Inc.
- 规格:
50 µg
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文献和实验2. Rakotobe, D. et al., Virol. J. 5, 32 (2008). Application Reference:
Pint lincRNA connects the p53 pathway with epigenetic silencing by the Polycomb repressive complex 2 (2013)
His-tag protein purification using Ni-NTA magnetic beads
His-tag protein purification using Ni-NTA magnetic beads Materials and reagents Extraction buffer 20 mM Tris-HCl, pH 8.0 1 mM PMSF 1 mg/ml lysozyme 0.05
Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Periplasmic Expression and Purification of Recombinant Fabs
fragments using immobilized metal-affinity chromatography via a pentahistidine (His) tag fused to the HC constant region 1 Fig. 1 ; 2 ). A similar modification to pComb3 (pComb3x) designed exclusively for the expression of soluble rFabs has been performed
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