- 询价
- BPS Bioscience
- 美国
- 40095
- 2025年08月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80°C (dry ice)
- 保质期：
At least 6 months at -80°C.
- 英文名：
CDK5/p35, GST-tag Recombinant
- 库存：
1
- 供应商：
BPS Bioscience Inc.
- 规格：
10 µg
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文献和实验2. Dhavan, R. et al. Nat Rev Mol Cell Biol. 2001 Oct,2(10):749-59.
Small Scale GST-Tag Purification Under Nature Conditions
of overloading). 3) Wash beads with 2x1.5ml PBS (washing: mix, spin 3min 3500rpm, keep supernatant aside). Keep sample of 40ul for PAGE-SDS of each washing . 4) Elute recombinant protein with 3 x 50ul elution buffer: 50mM TrisHCl pH8.0 10mM reduce
Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase
LubX is one such bacterial E3 ubiquitin ligase. We describe here the method to purify soluble LubX protein using GST-tag and Escherichia coli overexpression systems. Using the purified protein together with recombinant ubiquitin, E1, and E2 enzymes
V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
, the DCC has been used to study neurite outgrowth(12). We have previously used the DCC to elucidate the role of Rho family proteinsin the chemotactic response of BAC1.2F5 macrophages to CSF-1 (13). Wedescribe the use of this chamber to study the response
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