Stable at least 12 months from date of receipt, when stored as directed (-20°C)
英文名:
SBE Reporter Kit (TGFβ/SMAD signaling pathway)
库存:
1
供应商:
BPS Bioscience Inc.
CAS号:
/
规格:
500 rxns.
The SBE Reporter kit is designed for monitoring the activity of TGFβ/SMAD signaling pathway in the cultured cells. The kit contains transfection-ready SBE luciferase reporter vector, which is a TGFβ pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized SBE responsive element located upstream of a minimal promoter. The SBE reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
• Monitor TGFβ signaling pathway activity.
• Screen for activators or inhibitors of the TGFβ/ SMAD signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the TGFβ pathway.