- 询价
- BPS Bioscience
- 美国
- 50096
- 2025年11月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
room temp.
- 保质期：
12 months from date of receipt, when stored as directed
- 英文名：
HAT Stop Solution
- 库存：
1
- 供应商：
BPS Bioscience Inc.
- CAS号：
/
- 规格：
20 ml
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文献和实验. Results It was found that the difference in pH levels that were used did not have stop development. The only significant effect was that the lower pH levels caused the zebrafish to develop slower. This was observed over a period of 48 hours
(29:1 ratio; 40% stock solution) (Sigma) (for hot-stop PCR only; see Steps 1-22)Agarose (for duplex PCR only; see Steps 40-53)Ammonium persulfate (APS; 10%, w/v), freshly prepared (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)α-32PdCTP (10
Alternatively, the cells can be resuspended in I-20 + 2-ME with 150 µl added per well. An additional 50 µl of a 4X HAT solution can be added at 16-24 hours after fusion. 2. Add peritoneal cells to the fused cells at 2.5 x 104 cells per ml
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